Description
HEPES Buffer CAS 7365-45-9 Quick Details:
| Chemical Name: | HEPES Buffer |
| Other Name: | HEPES，Free acid; HEPES, Biological Buffer, Ultrapure; 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid; 4-Hydroxyethylpiperazine ethanesulfonic acid |
| CAS: | 7365-45-9 |
| EINECS: | 230-907-9 |
| Type: | Pharmaceutical raw materials; Pharmaceutical intermediates; Dye intermediates; Pesticide intermediates; Cosmetic raw materials |
| HEPES Molecular Formula: | C8H18N2O4S |
| HEPES Molecular Weight: | 238.3 |
HEPES Buffer CAS 7365-45-9 Chemical Properties:
| Melting point | 234-238 °C |
| density | 1.07 g/mL at 20 °C |
| refractive index | n20/D 1.339 |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| PH Range | 7.0 – 7.6 |
| HEPES pka | 7.5(at 25℃) |
| Water Solubility | Soluble |
| λmax | λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| HEPES Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
Usage:
Overview of HEPES Buffer
HEPES buffer (4-hydroxyethylpiperazineethanesulfonic acid) is a non-ionic amphoteric buffer. It is highly polar, inert to various chemical reagents and enzymes, and does not interfere with biochemical reactions. This makes it suitable for studying organelles and pH-sensitive proteins.
Applications in Cell Culture
HEPES buffer facilitates cell-cell adhesion and is used for short-term cell aggregation cultures, as well as for washing tissues and cells.
Research and Patents
Patent CN201310477071.6 describes a method for preparing porous zinc oxide microspheres using HEPES buffer as a template. This method is easy to execute, yielding microspheres with uniform size and hierarchical pore structures, which are useful in catalysts and gas sensing applications. Additionally, Patent CN201080052103.2 explores HEPES buffer and its derivatives for treating cancer pain and mitigating chemotherapy side effects.
Safety and Regulatory Approval
HEPES is a common component in animal and human cell culture buffers. Studies indicate it has minimal cytotoxicity compared to other buffers. Furthermore, the FDA has approved piperazine-based zwitterionic molecules as food additives, highlighting its safety in various applications.
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